Chapter 5 Oxygen Transfer in Bioreactors


Oxygen is needed by cells for respiration. Oxygen used by cells in suspension must be available as dissolved oxygen. Since oxygen solubility is quite small, about 6 to 7 mg/L under normal cultivation conditions, metabolic oxygen requirement is supplied on a as needed basis by continuous aeration of culture medium. Actively respiring yeast requires about 0.15 g O2 (g cell)-1 h. At a cell concentration of 10 g L-1, medium saturated with air can support less than 30 seconds worth of metabolic oxygen. That is, a continuous supply of oxygen must be maintained in any viable aerobic manufacturing process. In this Chapter, we will first get a quantitative appreciation for metabolic oxygen demand, followed by methods used in calculating rates at which oxygen is transfered from sparged air. We will then examine methods useful in characterizing oxygen mass transfer coefficient. Finally we will evaluate bioreactor operation and design based on oxygen transfer capability.



5.1 Metabolic Oxygen Demand
5.2 Volumetric Oxygen Mass Transfer Coefficient
5.3 Bioreactor Oxygen Balance
5.4 Factors Affecting KLa
5.5 Measurement of KLa
5.6 Scale-up Design Considerations
5.7 Case Studies