Chapter 5 Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (or PCR) was invented by Kary Mullis as a method to amplify a specific sequence of DNA millions of times in the test tube. Thus, we may think of PCR as an in vitro technique for gene cloning. Instead of plasmids replicating inside of bacteria, a DNA template, suitable primers and substrates together with a special DNA polymerase carry out the process in a test tube. Often the DNA from a single cell--such as a single hair or sperm is sufficient to carry out the procedure.