Table of Contents

• 1 Introduction
• 2 Molecular Biology
  • 2.1 Properties of Nucleic Acids
  • 2.2 Proteins and Amino Acids
  • 2.3 Making an RNA Copy of DNA
  • 2.4 The Genetic Code for Protein Synthesis
  • 2.5 Making a Genetic Probe
  • 2.6 The Control Regions of Genes
• 3 Cloning Genes
  • 3.1 Overview
  • 3.2 Cutting DNA
  • 3.3 Joining DNA: DNA Ligase
  • 3.4 Host Strains
  • 3.5 Cloning Vectors for E. coli
  • 3.6 Plasmid Cloning Vectors for E. coli
  • 3.7 Transforming Cells with Plasmids
  • 3.8 Selecting Transformed Cells
  • 3.9 Replica Plating for Colony Screening
  • 3.10 Nucleic Acid Probes
  • 3.11 Purifying Plasmid DNA
  • 3.12 Colored Indicators
  • 3.13 Let's Clone!
    • Stomatostatin
    • Insulin
• 4 More Advanced Cloning Techniques
  • 4.1 Bacteriophage l Vectors
  • 4.2 Cosmid Vectors
  • 4.3 Finding Genes: DNA Libraries
    • cDNA Libraries
    • Genomic Libraries
  • 4.4 More Cloning
• 5 Polymerase Chain Reaction (PCR)
  • 5.1 The PCR Reaction
  • 5.2 Reaction Particulars
• 6 Insulin and Diabetes
  • 6.1 Insulin
  • 6.2 Diabetes
• References