Table of Contents

• 1 Introduction
  • 1.1 What is a Bioreactor?
  • 1.2 Production and Purification
  • 1.3 Bioreactor Engineering Issues
• 2 Stoichiometry of Cellular Growth
  • 2.1 Cell Composition
  • 2.2 Growth Reaction
  • 2.3 Cell Yield and Stoichiometric Coefficents
  • 2.4 Mathematical Definition of Yield
  • 2.5 Measurement of Stoichiometric Coefficients
• 3 Thermodynamics of Cellular Growth
  • 3.1 Heat Release due to Growth
  • 3.2 Heat of Combustion Data
  • 3.3 Experimental Observations
  • 3.4 Heat Release when Extracellular Products are Formed
• 4 Kinetics of Growth and Product Formation
  • 4.1 Growth Kinetics
  • 4.2 What Does µ Depend on?
  • 4.3 Rate Expression and Metabolic Quotient
  • 4.4 Factors Affecting Growth Rate
  • 4.5 Product Formation Kinetics
• 5 Oxygen Transfer in Bioreactors
  • 5.1 Metabolic Oxygen Demand
  • 5.2 Volumetric Oxygen Mass Transfer Coefficient
  • 5.3 Bioreactor Oxygen Balance
  • 5.4 Factors Affecting KLa
  • 5.5 Measurement of KLa
  • 5.6 Case Studies